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Text File
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1996-07-05
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6KB
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172 lines
BlueScribe - Cloning vector
ENTRY BLUE #TYPE DNA CIRCULAR
TITLE BlueScribe - Cloning vector
DATE 03-MAR-1986
#sequence 16-DEC-1986
#sequence 02-FEB-1987
#sequence 04-MAR-1987
ACCESSION VB0042
SOURCE artificial
REFERENCE
#number 1
#authors Fernandez J.M., Short J.M., Renshaw M., Huse W., Sorge J.
#journal Gene (1987) in press
#citation sequence data from StrataGene
#comment sequence correction according to Stratagene
COMMENT
Obtained 28-JAN-1987 from StrataGene on floppy disc.
Revised 2-FEB-1986 by F. Pfeiffer:
1122/3 "AT" to "TA" to match revised sequence of PBR322
Revised 4-MAR-1987 to match sequence of pUC19 on request
of Stratagene
COMMENT
The stand shown corresponds to pUC19c.
As in the published sequence of pUC19c, The M13mp19 lacZ region
is on the complementary strand.
KEYWORDS
CROSSREFERENCE
#parent
VecBase(3):pUC19, VecSource(3):PromT7, VecSource(3):PromT3
#offspring
VecBase(3):BlueM13p, VecBase(3):BlueM13m
PARENT
Features of BlueScribe (2746 bp)
residue source
1- 400 684- 281 (c) pUC19
402- 421 1- 20 T7 promoter
423- 479 1- 57 M13mp19/pUC19-polylinker
485- 506 20- 1 (c) T3 promoter
508- 744 237- 1 (c) pUC19
745-2746 2686- 685 (c) pUC19
Conflict (cfl) and Mutations (mut): none
PARENT
Features of BlueScribe (2746 bp)
residue source
1- 400 1- 400 pUC19c
402- 421 1- 20 T7 promoter
423- 479 57- 1 (c) M13mp19/pUC19-polylinker
485- 506 20- 1 (c) T3 promoter
513-2746 453-2686 pUC19c
Conflict (cfl) and Mutations (mut): none
FEATURE
1689-2477 789-1 (c) Ap-R; b-lactamase
POLYLINKER HindIII-SphI-PstI-SalI-XbaI-BamHI-SmaI-KpnI-SacI-EcoRI
SELECTION
#resistance Ap
#indicator beta-galactosidase
SUMMARY Blue #length 2746 #checksum 2145
SEQUENCE
VECBASE:BLUE Length: 2746 May 7, 1989 20:09 Check: 2145 ..
1 TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG
51 GAGACGGTCA CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG
101 TCAGGGCGCG TCAGCGGGTG TTGGCGGGTG TCGGGGCTGG CTTAACTATG
151 CGGCATCAGA GCAGATTGTA CTGAGAGTGC ACCATATGCG GTGTGAAATA
201 CCGCACAGAT GCGTAAGGAG AAAATACCGC ATCAGGCGCC ATTCGCCATT
251 CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC TCTTCGCTAT
301 TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA
351 ACGCCAGGGT TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGAATT
401 GTAATACGAC TCACTATAGG GCGAATTCGA GCTCGGTACC CGGGGATCCT
451 CTAGAGTCGA CCTGCAGGCA TGCAAGCTTT TGTTCCCTTT AGTGAGGGTT
501 AATTCCGAGC TTGGCGTAAT CATGGTCATA GCTGTTTCCT GTGTGAAATT
551 GTTATCCGCT CACAATTCCA CACAACATAC GAGCCGGAAG CATAAAGTGT
601 AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA TTGCGTTGCG
651 CTCACTGCCC GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT
701 GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC
751 GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC
801 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG
851 ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC
901 CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA
951 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG
1001 GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT
1051 CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC
1101 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG
1151 TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG
1201 CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT
1251 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA
1301 GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC
1351 TACGGCTACA CTAGAAGGAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC
1401 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA
1451 CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA
1501 AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC
1551 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA
1601 AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA
1651 ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT
1701 CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG
1751 CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT
1801 GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA
1851 TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC
1901 CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT
1951 AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC
2001 TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT
2051 CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA
2101 AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC
2151 CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG
2201 TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG
2251 TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC
2301 AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA
2351 TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG
2401 AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC
2451 TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG
2501 CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC
2551 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG
2601 CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC
2651 GCACATTTCC CCGAAAAGTG CCACCTGACG TCTAAGAAAC CATTATTATC
2701 ATGACATTAA CCTATAAAAA TAGGCGTATC ACGAGGCCCT TTCGTC